Impact of Low Lithium Concentrations on the Fatty Acids and Elemental Composition of Salvinia natans.

The photosynthetic pigments, protein, macro and microelements concentrations, and fatty acids composition of Salvinia natans, a free-floating aquatic plant, were analyzed after exposure to Hoagland nutrient solution containing 1, 3, and 5 mg/L Li. The Li content of Salvinia natans grew exponentially with the Li concentration in the Hoagland nutrient solution. The exposure to Li did not induce significant changes in Na, Mg, K, Cu, and Zn content but enhanced the Ba, Cr, Mn, Ni and Mo absorption in Salvinia natans. The most abundant fatty acids determined in oils extracted from Salvinia natans were C16:0, C18:3(n6), C18:2(n6), and C18:3(n3). The photosynthetic pigments did not change significantly after exposure to Li. In contrast, chlorophyll and protein content decreased, whilst monounsaturated and polyunsaturated fatty acids content increased after the exposure to 1 mg/L Li. The results indicated that Salvinia natans exposed to low Li concentrations may be a good source of minerals, omega 6 and omega 3.

Lithium Accumulation in Salvinia natans Free-Floating Aquatic Plant.

The new context of the intensive use of lithium-based batteries led to increased production of Li and Li-containing wastes. All these activities are potential sources of environmental pollution with Li. However, the negative impact of Li on ecosystems, its specific role in the plants' development, uptake mechanism, and response to the induced stress are not fully understood. In this sense, the Li uptake and changes induced by Li exposure in the major and trace element contents, photosynthetic pigments, antioxidant activity, and elemental composition of Salvinia natans were also investigated. The results showed that Salvinia natans grown in Li-enriched nutrient solutions accumulated much higher Li contents than those grown in spring waters with a low Li content. However, the Li bioaccumulation factor in Salvinia natans grown in Li-enriched nutrient solutions was lower (13.3-29.5) than in spring waters (13.0-42.2). The plants exposed to high Li contents showed a decrease in their K and photosynthetic pigments content, while their total antioxidant activity did not change substantially.

In Vitro Digestibility of Minerals and B Group Vitamins from Different Brewers' Spent Grains.

Brewers' spent grain (BSG), the main by-product of the brewing industry, is a rich source of minerals and water-soluble vitamins such as thiamine, pyridoxine, niacin, and cobalamin. Bioaccessibility through in vitro digestion is an important step toward the complete absorption of minerals and B group vitamins in the gastrointestinal system. Inductively coupled plasma optical emission spectrometry (ICP-OES) together with inductively coupled plasma quadrupole mass spectrometry (ICP-MS) was used for the quantification of the macro- and micro-minerals. An ultra-high performance liquid chromatography (UHPLC) system coupled with a diode array detector (DAD) was used for B group vitamin identification. Four different industrial BSG samples were used in the present study, with different percentages of malted cereals such as barley, wheat, and degermed corn. Calcium's bioaccessibility was higher in the BSG4 sample composed of 50% malted barley and 50% malted wheat (16.03%), while iron presented the highest bioaccessibility value in the BSG2 sample (30.03%) composed of 65% Pale Ale malt and 35% Vienna malt. On the other hand, vitamin B1 had the highest bioaccessibility value (72.45%) in the BSG3 sample, whilst B6 registered the lowest bioaccessibility value (16.47%) in the BSG2 sample. Therefore, measuring the bioaccessibilty of bioactive BSG compounds before their further use is crucial in assessing their bioavailability.

Exploring the Properties of Micronized Natural Zeolitic Volcanic Tuff as Cosmetic Ingredient.

This study explores the characteristics of a micronized natural zeolitic volcanic tuff (MZ) as ingredient in cosmetic formulations. In particular, the purpose was to prepare and investigate the organoleptic and physicochemical properties of two representative cosmetic formulations containing MZ. The MZ samples were characterized using X-ray fluorescence (XRF), X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy. pH, cation exchange capacity (CEC), apparent density, chemical composition and particle size distribution of MZ samples were also determined. The micronization treatment applied did not produce significant structural and physicochemical changes with respect to the raw zeolitic volcanic tuff. The prepared formulations containing 5% MZ with different particle sizes (100-125 µm and 125-250 µm) were subjected to preliminary and accelerated stability tests, and the pH and organoleptic properties were also evaluated. The cosmetic formulations presented a pH of 4.3, a pleasant touch, good spreadability, easy application on skin, no color alteration and a good stability after 15, 30 and 60 days of storage at room temperature, low temperature and freezer during the accelerated stability tests. The obtained results endorse the MZ as suitable for the development of formulations exploiting the clinoptilolite properties as a cosmetic ingredient.

Effect of in vitro simulated gastrointestinal digestion on some nutritional characteristics of several dried fruits.

To provide new information on potential health benefits of dried fruits, the effects of in vitro digestion on minerals, total phenolics, total sugars and the antioxidant capacities were evaluated. For the first time, the bioaccessibility of these compounds during in vitro digestion was assessed by multivariate statistical analysis. Although the amount of all minerals decreased after digestion, moderate bioaccessibility was found, excepting Zn. The highest bioaccessibility of phenolics was obtained in prunes and the lowest in dates and cranberries. Total sugars content increased after in vitro digestion of dates, raisins and coconut, but decreased for cranberries, prunes and banana. The in vitro digestion led to an increase in the antioxidant capacity for the majority of dried fruits. The similarities/dis-similarities in pattern of analyzed parameters during digestion was revealed by heat map. Two-way joining cluster and principal component analysis were used to highlight the most relevant parameters in each digestion phase.

Application of Inductively Coupled Plasma Spectrometric Techniques and Multivariate Statistical Analysis in the Hydrogeochemical Profiling of Caves-Case Study Cloșani, Romania.

The aim of the study was to develop the hydrogeochemical profiling of caves based on the elemental composition of water and silty soil samples and a multivariate statistical analysis. Major and trace elements, including rare earths, were determined in the water and soil samples. The general characteristics of water, anions content, inorganic and organic carbon fractions and nitrogen species (NO3- and NH4+) were also considered. The ANOVA-principal component analysis (PCA) and two-way joining analysis were applied on samples collected from Cloșani Cave, Romania. The ANOVA-PCA revealed that the hydrogeochemical characteristics of Ca2+-HCO3- water facies were described by five factors, the strongest being associated with water-carbonate rock interactions and the occurrence of Ca, Mg and HCO3- (43.4%). Although organic carbon fractions have a lower influence (20.1%) than inorganic ones on water characteristics, they are involved in the chemical processes of nitrogen and of the elements involved in redox processes (Fe, Mn, Cr and Sn). The seasonal variability of water characteristics, especially during the spring, was observed. The variability of silty soil samples was described by four principal components, the strongest influence being attributed to rare earth elements (52.2%). The ANOVA-PCA provided deeper information compared to Gibbs and Piper diagrams and the correlation analysis.

Effect on Nutritional and Functional Characteristics by Encapsulating Rose canina Powder in Enriched Corn Extrudates.

Wild Rose canina fruit represents a rich source of bioactive compounds such as minerals, phenolic compounds, vitamins, carotenoids, folate, and antioxidant activity that still needs to be further exploited. Thus, this study aimed to use wild Rosa canina fruit encapsulated powder with different biopolymers aiming to manufacture ready-to-eat products, such as corn extrudates. To achieve this goal, extrudate physicochemical characteristics, such as water content (xw), water activity (aw), water absorption index (WAI), water solubility index (WSI), swelling index (SWE), hygroscopicity (Hy), expansion index (SEI), bulk density (ρb), porosity (ε), textural, optical; nutritional; and functional analysis (phenolic acids, flavonoids, ascorbic and dehydroascorbic acids, vitamin C, carotenoids, folates, antioxidant activity, and minerals) were determined. Results highlighted that 4 and 8% addition of wild Rose canina fruit encapsulated powder could be successfully used in the corn extrudates, showing the positive influence on its nutritional and functional value. Strong positive Pearson correlations were identified between antioxidant capacity and total flavonoids, carotenoids, folates, and vitamin C of mixtures and extrudates Minerals increased their amount during the extrusion process, reaching the highest values at an addition of 8% rosehip encapsulated with pea protein biopolymer. Furthermore, from the biopolymers used in the present study, pea protein powder exhibited the highest protection on the analyzed bioactive compounds against the extrusion process.

Immobilization of Potentially Toxic Elements in Contaminated Soils Using Thermally Treated Natural Zeolite.

Rehabilitation of contaminated soils is a complex and time-consuming procedure. One of the most cost-effective and easy-to-use soil remediation approaches is the use of amendments that stabilize the potential toxic elements (PTE) in soil by reducing their mobility and bioavailability. The stabilization of Cu, Pb, Zn, Cd, Co, Cr, Ni in a contaminated soil using 5% and 10% amendment with thermally treated natural zeolite was investigated using a sequential extraction procedure, contamination and environmental risk factors. The results showed that after amendment, the PTE concentration decreased in the exchangeable and reducible fractions and increased in the oxidizable and residual fractions. The highest immobilization effect, consisting in the decrease of exchangeable fractions with 69% was obtained in case of 10% zeolite amendment and 90 days of equilibration time for Pb; also, more than half of the mobile fraction was immobilized in case of Zn, Cu, and Co and about one third in case of Ni, Cr, and Cd. Generally, the immobilization effect of the 5% and 10% amendment is comparable, but a higher equilibration time enhanced the immobilization effect, especially in the case of Cd, Co, Cu, Pb, and Zn.

Assessment of Lithium, Macro- and Microelements in Water, Soil and Plant Samples from Karst Areas in Romania.

Lithium is a critical element for the modern society due to its uses in various industrial sectors. Despite its unequal distribution in the environment, Li occurrence in Romania was scarcely studied. In this study a versatile measurement method using ICP-MS technique was optimized for the determination of Li from various matrixes. Water, soil, and plant samples were collected from two important karst areas in the Dobrogea and Banat regions, Romania. The Li content was analyzed together with other macro- and microelement contents to find the relationship between the concentration of elements and their effect on the plants' Li uptake. In Dobrogea region, half of the studied waters had high Li concentration, ranging between 3.00 and 12.2 µg/L in the case of water and between 0.88 and 11.1 mg/kg DW in the case of plants, while the Li content in the soil samples were slightly comparable (from 9.85 to 11.3 mg/kg DW). In the Banat region, the concentration of Li was lower than in Dobrogea (1.40-1.46 µg/L in water, 6.50-9.12 mg/kg DW in soil, and 0.19-0.45 mg/kg DW in plants). Despite the high Li contents in soil, the Li was mostly unavailable for plants uptake and bioaccumulation.

Use of Black Poplar Leaves for the Biomonitoring of Air Pollution in an Urban Agglomeration.

Trees are considered to be an effective tool for metal pollution biomonitoring. In the present study, the concentration of metals (Cu, Pb, Zn, Cd, Co, Ni, Fe, Mn, and Al) in black poplar leaves (Populus nigra L.), together with the concentration of PM10, PM2.5, PM1, and total suspended particles (TSP), was used for the air pollution biomonitoring in 12 sites from various areas of Cluj-Napoca city, Romania. The concentration of PM10 in the air was high, but their metal content was low. However, Cu, Pb, and Zn were moderately enriched, while Cd was highly enriched in PM10 due to anthropogenic sources. The average metal concentration in leaves decreased in the order Zn>>Fe>Mn>Al>Pb>Ni>Cu>Co>Cd and increased with the increase of PM10 concentration, indicating that poplar leaves are sensitive to air pollution. The principal component analysis indicated that traffic, waste burning, road dust resuspension, and soil contamination are the main anthropogenic sources of metals in poplar leaves. The results indicated that black poplar leaves are a suitable biomonitoring tool for metal pollution, in urban environments.

Health Risk Assessment in Southern Carpathians Small Rural Communities Using Karst Springs as a Drinking Water Source.

The chemical quality of waters from eight karst springs from the Southern Carpathians and the health risk of small rural communities using these springs as a drinking water source were assessed. The results indicated that the spring waters in the studied area are chemically suitable to be used as drinking water and pose no health risks for adults and children. The spring water can be generally described as having circumneutral pH, Ca-Mg-HCO3- facies, excellent to good palatability, and low trace metal and nitrate content. The variation of chemical parameters between spring and autumn was low. These springs could become appropriate drinking water sources for the neighboring rural communities after the assessment of their microbiological status and, if it is the case, proper water treatment. Moreover, periodic monitoring of the water's chemical parameters, mostly nitrates, as well as the establishment of a protected area near the springs to prevent the negative impact of anthropogenic sources on water quality is recommended.